HAEAMBIO Co., Ltd. leverages its network with leading hospitals and research institutions both domestically and internationally to identify new targets for heart disease treatment. Based on this foundation, we have secured core technologies such as patient-derived valve cell isolation, gene profiling, and the discovery and validation of calcification-related genes.
Core Technology IEstablishment of a Valve Tissue Bank for Aortic Valve Disease Research
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IRB Approval: Seoul St. Mary's Hospital, St. Vincent's Hospital, Incheon St. Mary's Hospital
Core Technology II Technology for Isolation of Patient-Derived Valve Interstitial Cells (VIC)
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1. Valve tissue
2. Incubation with collagenase at 37°C in a CO2 incubator for 5 minutes
3. Scraping VEC with a sterile swab
4. Further incubation at 37°C in a CO2 incubator for 12-18 hours
Day 0
Day 4
Day 6
Core Technology III Gene Profiling of Patient-Derived Valve Interstitial Cells (VIC)
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Core Technology IV Acquisition of Single-Cell Sequencing Data from Patient-Derived Heart Valves
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Single-cell RNA Sequencing of Human Valve Tissues
patient
XXX (M/69)
XXX (M/68)
XXX (M/70)
XXX (M/46)
XXX (M/73)
XXX (M/59)
XXX (M/46)
tissue images
Classification
Bicuspid(NCC)
Tricuspid(NCC)
Tricuspid(NCC)
Bicuspid(NCC)
Bicuspid(RCC+NCC)
AR
Nomal(RCC+NCC_LCC)
Core Technology V Identification and Validation of Target Genes Related to Calcification
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Core Technology VIDevelopment of an Animal Model for Valve Calcification
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New Zealand White Rabbit
Anesthetizing and fixing rabbits in the anatomical position
Heparin infusion through the ear vein, exposure of the carotid artery
Insertion of a vascular guide wire through the carotid artery
Balloon catheter insertion
Injection of contrast agent
Inflating the balloon multiple times under imaging guidance to induce injury
Alizarin Red S
Core Technology VIIEvaluation of Therapeutic Efficacy Using the Valve Calcification Animal Model